Surface Plasmon Resonance (SPR) has emerged as a sensitive, real-time and label free technique for quantifying biomolecular interactions.[1]By combining SPR with electrochemical methods, many new capabilities and applications are now possible. This
A major advantage of SPR technology is that the detection does not require the analyte to be labeled, such as with a fluorescent molecule or a redox-active tag. This is because SPR directly detects changes in refractive index resulting from changes
In 2008, the illegal adulteration of infant milk products with melamine (structure shown in FIG 1) led to an outbreak of adverse kidney and urinary effects in thousands of children in China.[1] Clandestine addition of melamine in milk products was t
Vascular endothelial growth factor (VEGF) is an angiogenic signal protein biomarker produced by oxygen-hungry cells to stimulate the growth of blood vessels.[1] It binds to specialized receptors on the surfaces of endothelial cells and directs them
Alzheimer’s disease (AD) is the most common neurodegenerative disorder, affecting over 6.5 million people over the age of 65 in the U. S. In the senile plaques of AD patient’s brain, the major components are peptides composed of 39–43 amino acid res
The complement system is an essential component of the human innate immune system, playing a critical role as a defense mechanism against invading pathogens, priming adaptive immune responses and helping to remove immune complexes and apoptotic cell
SPR is sensitive to various processes taking place on or near a sensor chip. The SPR sensor chip can also simultaneously serve as a working electrode for electrochemical measurements. Combining electrochemical with SPR measurements has led to the de
This application note describes the simultaneous SPR detection of wild-type and mutant p53 proteins in cancer cell laysates. p53 is a transcription factor (i.e., DNA-binding protein) that plays an important role in DNA repair and tumor suppression b